Table of Contents

Tittle:- Top 40 NEET Questions on Biotechnology and Its Applications:-

Introduction: Top 40 NEET Questions on Biotechnology and Its Applications

Biotechnology is a rapidly evolving field with vast applications in medicine, agriculture, and industry—making it a crucial topic for NEET aspirants. Mastering this subject not only boosts your score but also builds a solid foundation for future studies in life sciences. In this blog, we’ve compiled the Top 40 NEET Questions on Biotechnology and Its Applications tailored for NEET preparation. These multiple-choice questions cover key concepts, current trends, and important NCERT-based facts to help you practice effectively. Whether you’re revising or testing your knowledge, this curated question set will sharpen your understanding and give you a competitive edge.

Top 40 NEET Questions on Biotechnology and Its Applications

The genetically modified brinjal in India has been developed for:
A. Drought resistance
B. Enhancing mineral content
C. Enhancing shelf life
D. Insect resistance
✅ Answer: D. Insect resistance
Which kind of therapy was given in 1990 to a four-year-old girl with ADA deficiency?

Top 40 NEET Questions

A. Radiation Therapy
B. Gene Therapy
C. Chemotherapy
D. Immunotherapy
✅ Answer: B. Gene Therapy
The maximum number of existing transgenic animals is of:
A. Fish
B. Mice
C. Cow
D. Pig
✅ Answer: B. Mice
The process of RNA interference has been used in the development of plants resistant to:
A. Insects
B. Nematodes
C. Fungi
D. Viruses
✅ Answer: B. Nematodes
The organism whose genetic material has been altered using genetic engineering is called:
A. Genetically mutant organism
B. Genetically modern organism
C. Genetically modified organism
D. Genetically transferred organism
✅ Answer: C. Genetically modified organism
The critical research area of biotechnology is:
A. Providing the best catalyst in the form of improved organism
B. Genetically modifying crops
C. Developing new antibiotics
D. Enhancing photosynthesis
✅ Answer: A. Providing the best catalyst in the form of improved organism
ELISA stands for:
A. Enzyme Like Immuno-sorbent Assay
B. Enzyme-Linked Immuno-sorbent Assay
C. Enzyme-Linked Immuno-similar Assay
D. Enzyme-Linked Immuno-sorbent Array
✅ Answer: B. Enzyme-Linked Immuno-sorbent Assay.
In situ hybridization is used to:
A. Remove mutations from a chromosome
B. Remove plasmids from a cell
C. Clone a gene
D. Locate a gene on a chromosome
✅ Answer: D. Locate a gene on a chromosome
Polymerase Chain Reaction (PCR) is not used in:
A. Confirming presence of a pathogen during early infection
B. Identifying mutated genes in suspected cancer patients
C. Isolating the gene of interest from host DNA to be cloned
D. Detection of the presence of HIV in suspected AIDS patient
✅ Answer: C. Isolating the gene of interest from host DNA to be cloned.
Golden rice has been genetically engineered to meet human nutritional requirements of:
A. Vitamin A
B. Vitamin B
C. Vitamin C
D. Vitamin D
✅ Answer: A. Vitamin A
Bt toxin gene has been cloned from Bacillus thuringiensis and expressed in plants to provide resistance against:
A. Bacteria
B. Fungi
C. Nematodes
D. Insects
✅ Answer: D. Insects
Which of the following is not a product of recombinant DNA technology?
A. Human insulin
B. Interferon
C. Penicillin
D. Hepatitis B vaccine
✅ Answer: C. Penicillin
The first genetically engineered insulin was produced by:
A. Eli Lilly
B. Biocon
C. Genentech
D. Novo Nordisk
✅ Answer: C. Genentech
Which of the following is not a biotechnological application in agriculture?
A. Development of pest-resistant crops
B. Production of biofertilizers
C. Micropropagation
D. Use of chemical pesticides
✅ Answer: D. Use of chemical pesticides
Which of the following is a bioethical issue in biotechnology?
A. Gene therapy
B. Cloning
C. Genetic modification
D. All of the above
✅ Answer: D. All of the above
Which of the following is used as a vector in gene therapy?
A. Plasmid
B. Bacteriophage
C. Retrovirus
D. Cosmid
✅ Answer: C. Retrovirus
The term ‘biopiracy’ refers to:
A. Use of pirated software in biotechnology
B. Unauthorized use of biological resources
C. Theft of biotechnology equipment
D. Illegal cloning of organisms
✅ Answer: B. Unauthorized use of biological resources
Which of the following is not a feature of genetically modified crops?
A. Increased yield
B. Resistance to pests
C. Enhanced nutritional value
D. Reduced shelf life
✅ Answer: D. Reduced shelf life
Which of the following is a transgenic animal?
A. Cow producing human insulin
B. Sheep producing alpha-1-antitrypsin
C. Goat producing spider silk protein
D. All of the above
✅ Answer: D. All of the above

We are completed here Top 20/Top 40 NEET Questions on Biotechnology and Its Applications
Which of the following is not a step in the process of gene cloning?
A. Isolation of DNA
B. Insertion of DNA into host
C. Expression of gene
D. Translation of mRNA
✅ Answer: D. Translation of mRNA
Which of the following is not a product of recombinant DNA technology?
A. Human insulin
B. Interferon
C. Penicillin
D. Hepatitis B vaccine
✅ Answer: C. Penicillin
Golden rice has been genetically engineered to meet human nutritional requirements of:
A. Vitamin A
B. Vitamin B
C. Vitamin C
D. Vitamin D
✅ Answer: A. Vitamin A
Bt toxin gene has been cloned from Bacillus thuringiensis and expressed in plants to provide resistance against:
A. Bacteria
B. Fungi
C. Nematodes
D. Insects
✅ Answer: D. Insects
The first genetically engineered insulin was produced by:
A. Eli Lilly
B. Biocon
C. Genentech
D. Novo Nordisk
✅ Answer: C. Genentech
Which of the following is not a biotechnological application in agriculture?
A. Development of pest-resistant crops
B. Production of biofertilizers
C. Micropropagation
D. Use of chemical pesticides
✅ Answer: D. Use of chemical pesticides.
Which of the following is a bioethical issue in biotechnology?
A. Gene therapy
B. Cloning
C. Genetic modification
D. All of the above
✅ Answer: D. All of the above.
Which of the following is used as a vector in gene therapy?
A. Plasmid
B. Bacteriophage
C. Retrovirus
D. Cosmid
✅ Answer: C. Retrovirus
The term ‘biopiracy’ refers to:
A. Use of pirated software in biotechnology
B. Unauthorized use of biological resources
C. Theft of biotechnology equipment
D. Illegal cloning of organisms
✅ Answer: B. Unauthorized use of biological resources.
Which of the following is not a feature of genetically modified crops?

Top 40 NEET Questions Applications

A. Increased yield
B. Resistance to pests
C. Enhanced nutritional value
D. Reduced shelf life
✅ Answer: D. Reduced shelf life
Which of the following is a transgenic animal?
A. Cow producing human insulin
B. Sheep producing alpha-1-antitrypsin
C. Goat producing spider silk protein
D. All of the above
✅ Answer: D. All of the above
Which of the following is not a step in the process of gene cloning?
A. Isolation of DNA
B. Insertion of DNA into host
C. Expression of gene
D. Translation of mRNA
✅ Answer: D. Translation of mRNA
Which of the following is not a method of gene transfer in plants?
A. Agrobacterium-mediated transformation
B. Microinjection
C. Electroporation
D. Liposome-mediated transfer
✅ Answer: B. Microinjection
Which of the following is not a component of a bioreactor?
A. Agitator
B. Oxygen supply system
C. Heating system
D. DNA ligase
✅ Answer: D. DNA ligase
Which of the following is not a characteristic of a plasmid?
A. Circular DNA
B. Double-stranded
C. Found in eukaryotic cells
D. Can replicate independently
✅ Answer: C. Found in eukaryotic cells
Which of the following is not a feature of a good cloning vector?
A. Small size
B. High copy number
C. Single restriction site
D. Antibiotic resistance gene
✅ Answer: C. Single restriction site
Which of the following is not a method of gene therapy?
A. Ex vivo
B. In vivo
C. In vitro
D. All of the above
✅ Answer: C. In vitro
Which of the following is not a feature of a genetically modified organism?
A. Contains foreign DNA
B. Produced by natural selection
C. Created using recombinant DNA technology
D. Used in agriculture and medicine
✅ Answer: B. Produced by natural selection
Which of the following is not a method of DNA fingerprinting?
A. RFLP
B. PCR
C. ELISA
D. VNTR analysis
✅ Answer: C. ELISA
Which of the following is not a component of PCR?
A. DNA template
B. Primers
C. DNA ligase
D. Taq polymerase
✅ Answer: C. DNA ligase
Which of the following is not a feature of a good host cell for cloning?
A. Should be able to take up foreign DNA
B. Should have restriction enzymes
C. Should allow replication of foreign DNA
D. Should not degrade foreign DNA
✅ Answer: B. Should have restriction enzymes.